Cytotoxicity is the quality of being toxic to cells.
Cells exposed to a cytotoxic compound can respond in a number of ways. The
cells may undergo necrosis, in which they lose membrane integrity and die
rapidly as a result of cell lysis; they can stop growing and dividing; or they
can activate a genetic program of controlled cell death, termed apoptosis.
Cytotoxicity testing: measuring viable cells, dead cells,
and detecting mechanism of cell death.
Testing the effects of compounds on the viability of cells
grown in culture is widely used as a predictor of potential toxic effects in
whole animals. Among the several alternative assays available, measuring the
levels of ATP is the most sensitive, reliable, and convenient method for
monitoring active cell metabolism. However, recently developed combinations of
methods have made it possible to collect more information from in vitro
cytotoxicity assays using standard fluorescence and luminescence plate readers.
This chapter describes two assay methods. The first utilizes beetle luciferase
for measuring the levels of ATP as a marker of viable cells. The second more
recently developed multiplex method relies on selective measurement of three
different protease activities as markers for viable, necrotic, and apoptotic
cells. Data analysis from the measurement of three marker protease activities
from the same sample provides a useful tool to help uncover the mechanism of
cell death and can serve as an internal control to help identify assay
artifacts.
HOW I GOT CURED OF HERPES VIRUS.
ReplyDeleteHello everyone out there, i am here to give my testimony about a herbalist called Dr imoloa. i was infected with herpes simplex virus 2 in 2013, i went to many hospitals for cure but there was no solution, so i was thinking on how i can get a solution out so that my body can be okay. one day i was in the pool side browsing and thinking of where i can get a solution. i go through many website were i saw so many testimonies about dr imoloa on how he cured them. i did not believe but i decided to give him a try, i contacted him and he prepared the herpes for me which i received through DHL courier service. i took it for two weeks after then he instructed me to go for check up, after the test i was confirmed herpes negative. am so free and happy. so, if you have problem or you are infected with any disease kindly contact him on email drimolaherbalmademedicine@gmail.com. or / whatssapp --+2347081986098.
This testimony serve as an expression of my gratitude. he also have
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